The biopharmaceutical industry often uses biological clean rooms for microbial contamination control. In the biological clean room, these microorganisms are mostly composed of bacteria and fungi, with a particle size of more than 0.2 μm, and the common bacterial particle size is above 0.5 μm, and most of the clothing is on other substance particles. Bio-pollution channels are not only related to the air, but also to the human body and to the clothing of the operators.